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Staff Profile

Dr Claudia Schneider

Principal Research Associate

  • Address: Biosciences Institute
    Â鶹´«Ã½ Centre for Cancer
    Faculty of Medical Sciences
    3rd Floor Cookson Building
    Â鶹´«Ã½
    Â鶹´«Ã½ upon Tyne,
    NE2 4H
Background
 
Qualifications
PhD in Biochemistry with Prof. Reinhard Lührmann (Philipps-University Marburg, Germany)
Previous Positions
Royal Society University Research Fellow (2011-2019)
Postdoctoral research with Prof. David Tollervey (Wellcome Trust Centre for Cell Biology, University of Edinburgh). During this time I held independent Fellowships from the Human Frontier Science Program Organisation and the European Molecular Biology Organisation.
Memberships
RNA Society, Biochemical Society
Honours and Awards
2011-2019       Royal Society University Research Fellowship (URF)
2005-2008       Human Frontier Science Program (HFSP) Long-Term Fellowship
2005                European Molecular Biology Organisation (EMBO) Long-Term Fellowship
2004                Prize for the best PhD at the Faculty of Medicine, Philipps-University Marburg, Germany
Research


Research Interests

Messenger RNAs (mRNAs) are the blueprints for protein production, but other types of RNA also drive and modulate all aspects of gene expression. In eukaryotic cells, RNA is never made “ready to use“, and the generation of functional molecules requires a sophisticated network of ribonucleases and co-factors that can mature the RNA. Ribonucleases are also important players in (m)RNA quality control networks, which monitor RNA processing pathways, maintain accurate RNA levels, and recognise and remove faulty molecules.

In many cases it is unclear how target RNAs are recognised and either processed or degraded, and exonucleases were long believed to be the main players. However, this view was challenged by the identification of a group of endonucleases containing PIN (PilT N-terminus) domains, which play key roles in RNA processing and quality control.

We are particularly interested in PIN domain endonucleases involved in RNA maturation, which often requires multiple processing steps. The assembly of ribosomes, large RNA-protein (RNP) machineries that synthesise all cellular proteins, is the major consumer of cellular energy and by far the most complex RNA processing pathway. Ribosome biogenesis determines the proliferative rate of cells and defects are linked to human diseases (“ribosomopathies”) and cancer. Key events in ribosomal RNA (rRNA) processing are endonucleolytic cleavages that release mature rRNAs from a large precursor transcript. Here, three PIN domain proteins are required for several RNA cleavage events. Using a combination of in vitro and in vivo approaches, we aim to characterise the roles of these PIN domain proteins and the timely and spatial regulation of their activity in budding yeast (Saccharomyces cerevisiae) and human cells.

Using budding yeast, we also study the role of a putative PIN domain endonuclease in the nonsense-mediated decay (NMD) messenger (m)RNA surveillance pathway, which degrades faulty mRNAs that could be translated into toxic proteins. Defects in the NMD pathway are linked to ~30% of all inherited human diseases (e.g. Duchenne muscular dystrophy) and cancer. This project also examines the intriguing, but not well understood link between mRNA quality control and genome stability.

Characterising fundamental, evolutionarily conserved processes such as ribosome biogenesis and RNA quality control in two eukaryotic systems offers a strong basis to clarify underlying mechanisms of genetic diseases, cancer and ageing, and will therefore enable the identification of new therapeutic targets.

Google Scholar: 


Teaching

Module Leader of Undergraduate Module BGM3063 (“Biochemistry of Gene Expression”)


Contribution to MRes Module MMB8008 ("Chromosome Biology and Cell Cycle Control in Health and Disease")


Primary and co-supervision as well as marking of PhD, MRes and Undergraduate Final Year Projects (Module CMB3000)


Active member of PhD progression panels and the tutor-tutee mentoring program for UG and PG students and Post Docs at Â鶹´«Ã½

Publications

  • Articles

    • Eastham MJ, Pelava A, Wells GR, Lee JK, Lawrence IR, Stewart J, Deichner M, Hertle R, Watkins NJ, Schneider C. . Nucleic Acids Research 2023, 51(17), 9397-9414.
    • Eastham MJ, Pelava A, Wells GR, Watkins NJ, Schneider C. . Biomolecules 2023, 13(6), 898.
    • Aquino GRR, Krogh N, Hackert P, Martin R, Gallesio JD, van Nues RW, Schneider C, Watkins NJ, Nielsen H, Bohnsack KE, Bohnsack MT. . Nucleic Acids Research 2021, 49(7), 4066-4084.
    • Muller JS, Burns DT, Griffin H, Wells GR, Zendah RA, Munro B, Schneider C, Horvath R. . Life Science Alliance 2020, 3(8), e202000678.
    • Sloan KE, Knox AA, Wells GR, Schneider C, Watkins NJ. . RNA Biology 2019, 16(2), 196-210.
    • Choque E, Schneider C, Gadal O, Dez C. . Nucleic Acids Research 2018, 46(9), 4699-4714.
    • Delan-Forino C, Schneider C, Tollervey D. . PLoS Genetics 2017, 13(3), e1006699.
    • Wells GR, Weichmann F, Sloan KE, Colvin D, Watkins NJ, Schneider C. . Nucleic Acids Research 2017, 45(8), 4796-4809.
    • Delan-Forino C, Schneider C, Tollervey D. . Wellcome Open Research 2017, 2, 34.
    • Wells GR, Weichmann F, Colvin D, Sloan KE, Kudla G, Tollervey D, Watkins NJ, Schneider C. . Nucleic Acids Research 2016, 44(11), 5399-5409.
    • Pelava A, Schneider C, Watkins NJ. . Biochemical Society Transactions 2016, 44(4), 1086-1090.
    • Sloan KE, Bohnsack MT, Schneider C, Watkins NJ. . RNA 2014, 20(4), 540-550.
    • Leung E, Schneider C, Yan F, Mohi-El-Din H, Kudla G, Tuck A, Wlotzka W, Doronina VA, Bartley R, Watkins NJ, Tollervey D, Brown JD. . Nucleic Acids Research 2014, 42(16), 10698-10710.
    • Schneider C, Kudla G, Wlotzka W, Tuck A, Tollervey D. . Molecular Cell 2012, 48(3), 422-433.
    • Lebaron S, Schneider C, van Nues RW, Swiatkowska A, Walsh D, Böttcher B, Granneman G, Watkins NJ, Tollervey D. . Nature Structural & Molecular Biology 2012, 19(8), 744-753.
    • Schneider C, Leung E, Brown J, Tollervey D. . Nucleic Acids Research 2009, 37(4), 1127-1140.
    • Pertschy B, Schneider C, Gnädig M, Schäfer T, Tollervey D, Hurt E. . Journal of Biological Chemistry 2009, 284(50), 35079-35091.
    • Skružný M, Schneider C, Rácz A, Weng J, Tollervey D, Hurt E. . PLoS Biology 2009, 7(1), e1000008.
    • Pessa HKJ, Will CL, Meng X, Schneider C, Watkins NJ, Perala N, Nymark M, Turunen JJ, Luhrmann R, Frilander MJ. . Proceedings of the National Academy of Sciences of the United States of America 2008, 105(25), 8655-8660.
    • Schneider C, Anderson JT, Tollervey D. . Molecular Cell 2007, 27(2), 324-331.
    • Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R, Elbashir S, Tuschl T, Lührmann R. . RNA 2004, 10(6), 929-941.
    • Schneider C, Will CL, Brosius J, Frilander MJ, Lührmann R. . Proceedings of the National Academy of Sciences 2004, 101(26), 9584-9589.
    • Watkins NJ, Lemm I, Ingelfinger D, Schneider C, Hoßbach M, Urlaub H, Lührmann R. . Molecular Cell 2004, 16(5), 789-798.
    • Schneider C, Will CL, Makarova OV, Makarov EM, Lührmann R. . Molecular and Cellular Biology 2002, 22(10), 3219-3229.
    • Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC. . EMBO Journal 2001, 20(16), 4536-4546.
    • Will CL, Schneider C, Reed R, Lührmann R. . Science 1999, 284(2003), 2003-2005.

  • Editorial

    • Schneider C, Tollervey D. . Nature Structural & Molecular Biology 2014, 21(1), 17-18.

  • Reviews

    • Schneider C, Bohnsack KE. . Wiley Interdisciplinary Reviews RNA 2023, 14(4), e1766.
    • Schneider C, Tollervey D. . Trends in Biochemical Sciences 2013, 38(10), 485-493.
    • Sloan KE, Schneider C, Watkins NJ. . Biochemical Society Transactions 2012, 40(4), 850-855.