麻豆传媒

My principal research interests focus on the regulation of synaptic connectivity in health and disease. Efficient synaptic communication is fundamental for every brain process. Dynamic structural and functional changes at the synapse are essential for the formation and refinement of neuronal networks. Importantly, mutations in genes linked to synapse formation and maintenance are associated with diseases of neurodevelopmental origin including epilepsy and autism spectrum disorders (ASD). Consequently, it is essential to understand the mechanisms that modulate the growth and maintenance of synapses in the healthy and diseased brain.

Using a multidisciplinary approach combining cellular biology, live imaging, and electrophysiology techniques in rodent models, I have established how a major class of synaptic modulators, Wnt secreted proteins, function at the synapse and respond to neuronal activity in the developing and mature nervous system. My research has been fundamental in establishing how Wnts contribute to synaptic plasticity and synapse integrity. However, not all findings in rodent model systems can be directly translatable to humans. Therefore, it is crucial to develop and use alternative approaches which can address these fundamental differences. I have established an optimised system which preserves developing and mature human cortical tissue in culture for days to weeks. Utilising this in vitro translational technique, I now aim to study how dysfunction in pre- and postsynaptic proteins cause epilepsy and ASD in humans.

• Articles

  • Parrish RR, MacKenzie-Gray Scott C, Jackson-Taylor T, Grundmann A, McLeod F, Codadu NK, Calin A, Alfonsa H, Wykes RC, Voipio J, Trevelyan AJ. . Journal of Neuroscience 2023, 43(5), 685-692.
  • Martin-Flores N, Podpolny M, McLeod F, Workman I, Crawford K, Ivanov D, Leonenko G, Escott-Price V, Salinas PC. . Elife 2023, 12, RP89453.
  • Zaaimi B, Turnbull M, Hazra A, Wang Y, Gandara C, McLeod F, McDermott EE, Escobedo-Cousin E, Idil AS, Bailey RG, Tardio S, Patel A, Ponon N, Gausden J, Walsh D, Hutchings F, Kaiser M, Cunningham MO, Clowry GJ, LeBeau FEN, Constandinou TG, Baker SN, Donaldson N, Degenaar P, O'Neill A, Trevelyan AJ, Jackson A. . Nature Biomedical Engineering 2023, 7, 559-575.
  • McLeod F, Dimtsi A, Marshall AC, Lewis-Smith DJ, Thomas R, Clowry GJ, Trevelyan AJ. . Brain 2023, 146(3), 850-857.
  • McLeod F, Boyle K, Marzo A, Martin-Flores N, Moe T, Palomer E, Gibb A, Salinas PC. . Frontiers in Synaptic Neuroscience 2020, 12, 575863.
  • Redlingshöfer L, McLeod F, Chen Y, Camus MD, Burden JJ, Palomer E, Briant K, Dannhauser PN, Salinas PC, Brodsky FM. . PNAS 2020, 117(38), 23527-23538.
  • Ferrari M, Bernis M, McLeod F, Podpolny P, Coullery R, Casadei I, Salinas P, Rosso S. . Journal of Cell Science 2018, 131(13), jcs216101.
  • McLeod F, Bossio A, Sibilla S, Ciani L, Hannan S, Wilson G, Smart T, Gibb A, Salinas P. . Cell Reports 2018, 23, 1060-1071.
  • McLeod F, Marzo A, Galli S, Salinas P. . Journal of Visualized Experiments 2017, 128, e56153.
  • Marzo A, Galli S, Lopes D, McLeod F, Podpolny M, Segovia-Roldan M, Ciani L, Cacucci F, Gibb A, Salinas P. . Current Biology 2016, 26(19), 2551-2561.
  • Ciani L, Marzo A, Boyle K, Stamatakou E, Lopes D, Anane D, McLeod F, Rosso S, Gibb A, Salinas P. . Nature Communications 2015, 6, 8302.
  • McLeod F, Ganley R, Williams L, Selfridge J, Bird A, Cobb SR. . Neuroscience 2013, 231, 195-205.
  • Weng SM, McLeod F, Bailey ME, Cobb SR. . Neuroscience 2011, 180, 314-321.

• Review

  • McLeod F, Salinas P. . Current Opinion in Neurobiology 2018, 53, 90-95.