麻豆传媒

Research Positions

2023 - present Professor of Structural Microbiology, NUBI, 麻豆传媒

2019 - 2023  Theme Lead for Molecular Mechanisms of Life Theme, FMS, 麻豆传媒

2019 - 2023  Senior Lecturer of Macromolecular Crystallography, NUBI, 麻豆传媒

2012 - 2019    Lecturer of Macromolecular Crystallography, ICaMB, 麻豆传媒

2011 - 2012    Research Associate, Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, UK

2008 – 2011   Research Associate, Division of Molecular Biosciences, Department of Life Sciences, Imperial College London, UK

2005 – 2008   Research Associate, School of Crystallography, Birkbeck College/University College, London, UK

Education

2001 – 2005  PhD in Structural Biology, Henry Wellcome Trust Building for Human Genetics, University of Oxford and Open University. "Structural Studies of RNA-dependent RNA polymerases"                        Supervisors: Dr Jon Grimes and Prof Dave I Stuart

1993 -1999   BSc in Biochemistry, Science Faculty & Institute of Biomedical Sciences “Abel Salazar”, University of Porto, Portugal. Research Project: "Structure determination of transthyretin variants"

Research Awards

March 2022 - March 2025 - Research Grant MRC

October 2017 - October 2021 - Wellcome Trust Collaborative Award

November 2014 - November 2017 - New Investigator Research Grant, MRC

April 2013 - March 2014 - Royal Society Research Grant

March - June 2011 - Wellcome Trust Value In People Award

Oct 01 - Oct 2005 - PhD Fellowship (part of Human Frontier Science Program Research Grant)

As a structural biologist interested in proteins associated with pathogenicity and disease, I have recently established a C. difficile Structural Microbiology group focusing on detailed structural and functional characterisation of proteins involved in key pathogenicity pathways in this major human pathogen. Combining these approaches, we are interested in C. difficile spores, the dormant, resistant cell forms that are the transmission vehicle, as well as surface proteins and their role in infection.

Clostridioides difficile S-layer

 
C. difficile is now the most prevalent hospital acquired infection in the UK. These gram-positive bacteria have an outside para-crystalline layer (S-layer) that is presumed to act as a protective shield and that is thought to be implicated in virulence, host interaction and immune activation. We focus on understanding S-layer organisation, primarily by determining the structure of the major S-layer protein constituent, SlpA. This work is part of a Wellcome Trust Collaborative Award with colleagues from Sheffield and Glasgow universities.

We are also studying other cell wall proteins identified in C. difficile, aiming to understand their macromolecular details with our structural studies that complement work developed by our collaborators.

Sporulation engulfment machinery 

Work in the Salgado lab, awarded an MRC New Investigator Grant (Nov 2014), has identified two essential proteins involved in the early stages of sporulation (Crawshaw et al., 2014, Serrano et al., 2016). We have also contributed to elucidating details of the engulfment mechanism to start defining the complex machinery involved in this process, which we termed the engulfasome (Kelly and Salgado, 2019). We have recently been awarded a Research Grant from the MRC to continue our work to unravel the molecular mechanism of engulfment in C. difficile.

Supervisor:

PhD students

            - Victoria Burge

            - ellie Rockliff

MRes/MSci, UG projects and summer vacancies

Module leader:

Biomolecular Research in Health and Disease (MMB8018), MRes module

Lecturer:

Advanced Protein Analysis (BGM2062), BSc Biochemistry 

• Articles

  • Latimer-Smith M, Salgado PS, Forsyth I, Makeyev E, Poranen MM, Stuart DI, Grimes JM, El Omari K. . Scientific Reports 2024, 14(1), 23540.
  • Martins D, Nerber HN, Roughton CG, Fasquelle A, Barwinska-Sendra A, Vollmer D, Gray J, Vollmer W, Sorg JA, Salgado PS, Henriques AO, Serrano M. . Molecular Microbiology 2024, 122(2), 213-229.
  • Ormsby MJ, Vaz F, Kirk JA, Barwinska-Sendra A, Hallam JC, Lanzoni-Mangutchi P, Cole J, Chaudhuri RR, Salgado PS, Fagan RP, Douce GR. . PLoS Pathogens 2023, 19(6), e1011015.
  • Lanzoni-Mangutchi P, Banerji O, Wilson J, Barwinska-Sendra A, Kirk JA, Vaz F, O'Beirne S, Basle A, El Omari K, Wagner A, Fairweather NF, Douce GR, Bullough PA, Fagan RP, Salgado PS. . Nature Communications 2022, 13(1), 970.
  • Julius C, Salgado PS, Yuzenkova Y. . Nucleic Acid Research 2020, 48(13), 7298-7306.
  • Crawshaw AD, Basle A, Salgado PS. . Acta Crystallographica Section D: Structural Biology 2020, 76, 261-271.
  • Kelly A, Salgado PS. . Anaerobe 2019, 60, 102091.
  • Dembek M, Kelly A, Barwinska-Sendra A, Vollmer D, Biboy J, Gray J, Vollmer W, Salgado PS. . Molecular Microbiology 2018, 110(3), 390-410.
  • Dembek M, Willing SE, Hong HA, Hosseini S, Salgado PS, Cutting SM. . Frontiers in Microbiology 2017, 8, 1793.
  • Serrano M, Crawshaw AD, Dembek M, Monteiro JM, Pereria FC, Gomes de Pinho M, Fairweather NF, Salgado PS, Henriques AO. . Molecular Microbiology 2016, 100(1), 204-228.
  • Warren AJ, Crawshaw AD, Trincao J, Aller P, Alcock S, Nistea I, Salgado PS, Evans G. . Acta Crystallographica Section D: Biological Crystallography 2015, 71, 2079-2088.
  • Lin J, Soon-Hwan O, Jones R, Garnett JA, Salgado PS, Rusnakova S, Matthews S, Hoyer LL, Cota E. . Journal of Biological Chemistry 2014, M114, 547877.
  • Douse CH, Green JL, Salgado PS, Simpson PJ, Thomas JC, Langsley G, Holder AA, Tate EW, Cota E. . Journal of Biological Chemistry 2012, 287(44), 36968-36977.
  • Salgado PS, Yan R, Taylor JD, Buchnell L, Jones R, Hoyer L, Matthews SJ, Simpson P, Cota E. . Proceedings of the National Academy of Sciences 2011, 108(38), 15775-15779.
  • Salgado PS, Taylor JD, Cota E, Mathews SJ. . Acta Crystallographica. Section D: Biological Crystallography 2011, 67(p.1), 8-13.
  • Salgado PS, Yan R, Rowan F, Cota E. . Acta Crystallographica. Section F: Structural Biology and Crystallization Communications 2011, 67(4), 467-470.
  • Taylor JD, Zhou Y, Salgado PS, Patwardhan A, McGuffie M, Pape T, Grabe G, Ashman E, Constable SC, Simpson PJ, Lee W, Cota E, Chapman MR, Matthews SJ. . Structure 2011, 19(9), 1307-1316.
  • Poranen MM, Salgado PS, Koivunen MRL, Wright S, Bamford DH, Stuart DI, Grimes JM. . Nucleic Acids Research 2008, 36(20), 6633-6644.
  • Salgado PS, Laurila MRL, Makeyev EV, Bamford DH, Stuart DI, Grimes JM. . PLoS Biology 2006, 4(12), e434.
  • Salgado PS, Walsh MA, Laurila MRL, Stuart DI, Grimes JM. . Acta Biol Crystallographica D 2005, D61(1), 108-111.
  • Laurila MR, Salgado PS, Makeyev EV, Nettelship J, Stuart DI, Grimes JM, Bamford DH. . Journal of Structural Biology 2005, 149(1), 111-115.
  • Laurila MRL, Salgado PS, Stuart DI, Grimes JM, Bamford DH. . Journal of General Virology 2005, 86(2).
  • Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM. . Structure 2004, 12(2), 307-316.

• Review

  • Crawshaw AD, Serrano M, Stanley WA, Henriques AO, Salgado PS. . FEMS Microbiology Letters 2014, 358(2), 129-136.