麻豆传媒

Current Position

Senior Research Technician/ Lab Manager – Genomics Core Facility

Experience

I have been delivering genomic services to research and commercial settings since 2003. Did a lot of Sanger sequencing and then began Next Generation Sequencing (NGS) on Illumina’s first instrument, the Genome Analyzer IIx, which the University had from 2008 to 2013. For NGS I now use Illumina’s MiSeq, NextSeq, and the NovaSeq. I have been at the Genomics Core Facility from the beginning (2015/2016) and I am qualified on all our instrumentation. I provide specific training on the 10X Genomics platform and Laser Microdissection (LMD).

Qualifications

BSc (Hons) Biomedical Science, University of Sunderland 2000 (including one year’s work placement at MRC Building, Institute for the Health of the Elderly, 麻豆传媒 General Hospital, Westgate Road, 麻豆传媒 upon Tyne, UK).

Previous roles

Research Technician – Mitochondrial Group of Professor Patrick Chinnery: 2014 - 2015

Senior Research Technician – BHF group of Professor Bernard Keavney: 2006 - 2013

Molecular Biology Technician – Complement Genomics: 2003 - 2005

Other interests

Active member of NU TechNet and Race Equality Charter Self Assessment Team

Key Words

Genomics / Next-Generation sequencing / 10X/ Single-cell / Transcriptomics / Spatial Gene Expression / DNA / RNA / ddPCR/ Laser Microscopy/ Whole Genomes/ Exomes/ NU TechNet/ REC SAT/

• Articles

  • Baker KF, McDonald D, Hulme G, Hussain R, Coxhead J, Swan D, Schulz AR, Mei HE, MacDonald L, Pratt AG, Filby A, Anderson AE, Isaacs JD. . Nature Communications 2024, 15, 1063.
  • Dorgau B, Collin J, Rozanska A, Zerti D, Unsworth A, Crosier M, Hussain R, Coxhead J, Dhanaseelan T, Patel A, Sowden JC, FitzPatrick DR, Queen R, Lako M. . Nature Communications 2024, 15, 3567.
  • Dorgau B, Collin J, Rozanska A, boczonadi V, Molina MM, Unsworth A, Hussain r, Coxhead J, Dhanaseelan T, Armstrong L, Queen R, Lako M. . iScience 2024, 27(4), 109397.
  • Collin J, Hasoon MSR, Zerti D, Hammadi S, Dorgau B, Clarke L, Steel D, Hussain R, Coxhead J, Lisgo S, Queen R, Lako M. . Human Molecular Genetics 2023, 32(10), 1698-1710.
  • Danilenko M, Zaka M, Keeling C, Crosier S, Lyman S, Finetti M, Williamson D, Hussain R, Coxhead J, Zhou P, Hill RM, Hicks D, Rand V, Joshi A, Schwalbe EC, Bailey S, Clifford SC. . Acta Neuropathologica 2022, 144, 565–578.
  • Rozanska A, Cerna R, Queen R, Collin J, Zerti D, Dorgau B, Beh C, Davey T, Coxhead J, Hussain R, Alaama J, Steel D, Benvenisty N, Armstrong L, Parulekar M, Lako M. . Stem Cell Translational Medicine 2022, 11(4), 415-433.
  • Calabrese C, Pyle A, Griffin H, Coxhead J, Hussain R, Braund PS, Li L, Burgess A, Munroe PB, Little L, Warren HR, Cabrera C, Hall A, Caulfield MJ, Rothwell PM, Samani NJ, Hudson G, Chinnery PF. . PLoS Genetics 2022, 18(4), e1010068.
  • Jackson RM, Hatton CF, Spegarova JS, Georgiou M, Collin J, Stephenson E, Verdon B, Haq IJ, Hussain R, Coxhead JM, Mudhar H-S, Wagner B, Hasoon M, Davey T, Rooney P, Khan A, Ward C, Brodlie M, Haniffa M, Hambleton S, Armstrong L, Figueiredo F, Queen R, Duncan CJ, Lako M. . Stem Cell Reports 2022, 17(7), 1699-1713.
  • Hatton CF, Botting RA, Duenas ME, Haq IJ, Verdon B, Thompson BJ, Spegarova JS, Gothe F, Stephenson E, Gardner AI, Murphy S, Scott J, Garnett JP, Carrie S, Powell J, Khan CMA, Huang L, Hussain R, Coxhead J, Davey T, Simpson AJ, Haniffa M, Hambleton S, Brodlie M, Ward C, Trost M, Reynolds G, Duncan CJA. . Nature Communications 2021, 12(1), 7092.
  • Jardine L, Webb S, Goh I, Quiroga Londono M, Reynolds G, Mather M, Olabi B, Stephenson E, Botting RA, Horsfall D, Engelbert J, Maunder D, Mende N, Murnane C, Dann E, McGrath J, King H, Kucinski I, Queen R, Carey CD, Shrubsole C, Poyner E, Acres M, Jones C, Ness T, Coulthard R, Elliott N, O'Byrne S, Haltalli MLR, Lawrence JE, Lisgo S, Balogh P, Meyer KB, Prigmore E, Ambridge K, Jain MS, Efremova M, Pickard K, Creasey T, Bacardit J, Henderson D, Coxhead J, Filby A, Hussain R, Dixon D, McDonald D, Popescu D-M, Kowalczyk MS, Li B, Ashenberg O, Tabaka M, Dionne D, Tickle TL, Slyper M, Rozenblatt-Rosen O, Regev A, Behjati S, Laurenti E, Wilson NK, Roy A, Gottgens B, Roberts I, Teichmann SA, Haniffa M. . Nature 2021, 598, 327-331.
  • Collin J, Queen R, Darin Zerti D, Bojic S, Dorgau B, Moyse N, Molina MM, Yang C, Dey S, Reynolds G, Hussain R, Coxhead JM, Lisgo S, Henderson D, Joseph A, Rooney P, Ghosh S, Clarke L, Connon CJ, Haniffa M, Figueiredo F, Armstrong L, Lako M. . The Ocular Surface 2021, 21, 279-298.
  • Khasawneh RR, Kist R, Queen R, Hussain R, Coxhead J, Schneider JE, Mohun TJ, Zaffran S, Peters H, Phillips HM, Bamforth SD. . BMC Developmental Biology 2021, 21, 14.
  • Collin J, Queen R, Zerti D, Dorgau B, Georgiou M, Djidrovski I, Hussain R, Coxhead JM, Joseph A, Rooney P, Lisgo S, Figueiredo FC, Armstrong L, Lako M. . The Ocular Surface 2020, 19, 190-200.
  • Collin J, Queen R, Zerti D, Dorgau B, Hussain R, Coxhead J, Cockell S, Lako M. . Stem Cells (Durham) 2019, 37(5), 593-598.
  • Hussain R, Steel DH, Sandinha T, Stappler T, Heimann H, Wong D. . Retina 2019, 39, S133-S136.
  • Collin J, Zerti D, Queen R, SantoFerreira T, Bauer R, Coxhead J, Hussain R, Steel D, Mellough C, Ader M, Sernagor E, Armstrong L, Lako M. . Stem Cells (Durham) 2019, 37(5), 609-622.
  • Duncan CJA, Dinnigan E, Theobald R, Grainger A, Skelton AJ, Hussain R, Willet JDP, Swan DJ, Coxhead J, Thomas MF, Thomas J, Zamvar V, Slatter MA, Cant AJ, Engelhardt KR, Hambleton S. . Annals of the Rheumatic Diseases 2018, 77(5), 783-786.
  • Coxhead J, Kurzawa-Akanbi M, Hussain R, Pyle A, Chinnery P, Hudson G. . Neurobiology of Aging 2016, 38, 217.e1-217.e6.
  • Keogh MJ, Steele H, Douroudis K, Pyle A, Duff J, Hussain R, Smertenko T, Griffin H, Santibanez-Koref M, Horvath R, Chinnery PF. . Journal of Neurology 2015, 262(8), 1822-1827.
  • Jiang Y, Habibollah S, Tilgner K, Collin J, Barta T, Al-Aama JY, Tesarov L, Hussain R, Trafford AW, Kirkwood G, Sernagor E, Eleftheriou CG, Przyborski S, Stojkovic M, Lako M, Keavney B, Armstrong L. . Stem Cells Translational Medicine 2014, 3(4), 416-423.
  • Dickinson RE, Griffin H, Bigley V, Reynard LN, Hussain R, Haniffa M, Lakey JH, Rahman T, Wang XN, McGovern N, Pagan S, Cookson S, McDonald D, Chua I, Wallis J, Cant A, Wright M, Keavney B, Chinnery PF, Loughlin J, Hambleton S, Santibanez-Koref M, Collin M. . Blood 2011, 118(10), 2656-2658.
  • Seifer CM, Hussain R, Clarke M, Kenny RA. . Journal of the American Geriatrics Society 1999, 47, P321.

• Conference Proceedings (inc. Abstracts)

  • Bada WU, Miossec MJ, Hussain R, Rahman T, Topf A, Hall D, Santibanez-Koref M, Keavney B, Goodship J. . In: ESC Congress 2014. 2014, Barcelona, Spain: Oxford University Press.
  • Bada WU, Miossec MJ, Hussain R, Goodship J, Keavney B. . In: Frontiers in CardioVascular Biology 2014: Third Congress of the ESC Council on Basic Cardiovascular Science. 2014, Barcelona: Oxford University Press.
  • Griffin H, McDonald DO, Singh-Dang T, Dickinson R, Grainger A, Reynard L, Hussain R, Cant AJ, Gennery AR, Abinun M, Flood TJ, Collin MP, Loughlin J, Morgan NV, Santibanez-Koref M, Hambleton S. . In: 15th Biennial Meeting European Society for Immunodeficiency (ESID). 2012, Florence, Italy: Springer.
  • Tan HL, Topf A, Griffin H, Doza JP, Hall D, Hussain R, Eden J, Rahman T, Cunnington M, Goodship J, Keavney B. . In: British Cardiovascular Society Annual Conference and Exhibition. 2009, London, UK: BMJ Publishing Group.
  • Seifer CM, Brennan R, Hussain R, Clarke M, Kenny RA. Comparison of response to carotid sinus massage in elderly persons with and without raised intraocular pressure. In: Journal of the American Geriatrics Society. 1999, John Wiley & Sons, Inc.

• Letter

  • Lowes H, Robertson F, Pyle A, Hussain R, Coxhead J, Yarnall AJ, Burn D, Payne BAI, Santibanez-Koref M, Hudson G. . Movement Disorders 2020, 35(12), 2134-2136.