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Bioimaging Unit
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Leica M205

Modalities: upright, stereo, fluorescence

Overview

The M205 is a fluorescence capable stereo microscope with automated zoom and filter wheel. It can be equipped with 0.5, 1 or 2x objectives, allowing a zoom range from ~ 3x to 360x with focus stacking options post acquisition.

It is equipped with several illumination options:

  • base light for transmission
  • ringlight (on 1x objective)
  • fixed angle spotlights
  • Metal Halide lamp for fluorescence with a choice of filters for GFP, YFP or RFP

Image capture is via a colour camera using Las-X software.

Imaging Modes

  • Brightfield
  • Fluorescence
  • Both with optional focus stacking

Within our facility we have two M205 systems.

M205 (1) - Located in the the main facility will not do transmission.

M205 (2) - Located in the Cookson Building 4th floor is capable of fluorescence and transmission.

Specification

Objectives

Magnification

NA

Coverslips

Other Modes

Immersion

0.32  N/A      Air
1  N/A      Air
2  N/A      Air

 Filters (epi)

Cube

Example Fluorophores

Excitation

Dichroic

Emission

Part Number

Leica GFP2 GFP Pluc 460-500   5510 (LP)  
Leica YFP YFP 500-520   540-580  
Leica TXR Texas Red 540-580   610 (LP)  

Light Sources

  • Transmission lamp with adjustable angle
  • Ringlight with optional sidelighting
  • Fixed angle spotlights
  • Gooseneck (available upon request)
  • Metal Halide lamp for Fluorescence

Camera

  • DFC310FX (1.3MP CCD camera)

Location

Mitochondrial research group, 4th Floor Cookson building

Location for M205 (2)

Mitochondrial Reserach Group

4th Floor Cookkson Building
The Medical School
Framlington Place
Â鶹´«Ã½ Upon Tyne
NE2 4HH
UK

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use.